INDUCTION AND DEVELOPMENT OF
GardenGenetics® can provide mutation induction services (including
polyploidization) in both monocot and dicot plant species. Mutagenesis
techniques use chemical and/or radiological mutagens under extremely
controlled experimental conditions. Polyploid induction techniques
utilized include the use of natural and synthetic compounds which
temporarily disrupt the cellular cytoskeleton during mitosis.
Newly induced plant mutants (including polyploids) tend to grow
slowly and erratically, requiring careful isolation of mutant
tissue. GardenGenetics® has the expertise to recover and propagate
mutant tissue; to produce advanced lines of seed-propagated mutants;
and to determine the inheritance of the recovered mutants.
Mutation induction of vegetative material frequently creates
cytochimerae. This phenomenon occurs when a section or layer of
the treated plant tissues contains both mutant and non-mutant
tissue. Cytochimerae can exhibit all potential variations of chimeral
composition, and these variations may not be easily discerned
by eye. Please be aware that no research lab can guarantee stable
mutation induction or polyploidization without extensive grow-outs
of both vegetatively and seed-propagated populations. GardenGenetics® can provide these follow-up services at your request, including
verification of ploidy by flow cytometry.
GardenGenetics® can provide mutation induction and polyploidization
services in these kinds of plants:
- Annuals (seed- and vegetatively-propagated)
- Herbaceous and woody perennials Flowering trees
- Herbs, botanicals, medicinals, and natural product crops
- Florist crops
GardenGenetics® can use both seed and vegetative plant material
as germplasm sources for mutation or polyploidization. Where possible
following induction, we prefer to conduct seed-propagated grow-outs
of induced mutants polyploids prior to final selection. Grow-out
and selection follow-up services are available at your request.